Sulfide and ammonium in some industrial wastewaters need abolishing. The three-chamber microbial fuel cell (MFC), consisting of an oxic-cathode and an anoxic-cathode sharing same anode, was explored to accomplish simultaneous sulfide removal, nitrification, denitrification and electricity generation. It provided a promise of recovering electricity from wastewater containing sulfide and ammonium. The three-chamber MFC run with separate sulfide feeding and ammonium feeding showed preferable nitrogen removal and electricity generation performance as compared to that with a mixed sulfide/ammonium feeding. The desirable feeding sulfide concentration for this MFC was deemed as 193.6 +/- 0.8 mg S L-1 considering both pollutant removal and electricity generation performance. Temperature rise favored both pollutants removal and electricity generation but hampered elemental sulfur recovery. The suitable temperature for this MFC was found to be 30 degrees C, achieving a total nitrogen removal rate of 10.0 +/- 1.3 g N m(-3) d(-1), a sulfide removal rate of 206.5 +/- 1.9 g S m(-3) d(-1), a sulfur recovery efficiency of 28.9%, and an electricity generation of 428.0 +/- 26.2 C/batch cycle. The distribution of coulombs to both cathodes affected the performance of nitrogen removal and electricity generation.