This study investigated the migration law of ion release and adsorption in the flotation pulp of oxide zinc ore, and found that numerous different ions existed in the pulp. The total concentrations and major sources of various ions (Pb2+, Zn2+, Fe3+, Ca2+, Mg2+, SO42-, etc.), released from the minerals after grinding, were determined. The zeta potential tests indicated that a mixed- ion solution strongly influences the surface charge of the minerals. The flotation tests show that the migration law of ions is detrimental to the separation and concentration of minerals.