화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.62, 462-470, June, 2018
DOI10.1016/j.jiec.2018.01.029  Export Citation
Different organic components on silica hybrid matrices modulate the lipase inhibition by the glycerol formed in continuous transesterification reactions
Lucas S. Martin1, 2, Annie Ceron1, Pedro C. Oliveira1, Gisella M. Zanin2, and Heizir F. de Castro1, †
  • 1Engineering School of Lorena, University of São Paulo, Lorena, SP, 12602-810, Brazil
  • 2Chemical Engineering Department, State University of Maringá, Maringá, PR, 87020-900, Brazil
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Silica hybrid materials, functionalized via incorporation of three organic components:β-cyclodextrin (βCD), carboxymethyl-cellulose (CMC) and hydroxyethyl-cellulose (HEC) were synthesized by the sol- gel technique and results were compared to a well-defined matrix (silica-polyvinyl alcohol-SiO2-PVA) with respect to immobilizing Burkholderia cepacia lipase. The main objective was set to select organic components that can replace the PVA to obtain a hybrid composite with greater hydrophobic character, without compromising the remarkable features of SiO2-PVA. All the three selected compounds allowed obtaining matrices that presented similar textural and morphological properties and gave high activity (1451-1661 U g-1) and thermal stability (t1/2> 70 h) upon immobilization. Regarding glycerol affinity, all matrices had lower ability than SiO2-PVA to adsorb glycerol, with the SiO2-βCD matrix showing the lowest affinity due to the cyclic structure of the βCD. Transesterification reactions of palm kernel oil with ethanol mediated by B. cepacia immobilized on SiO2-βCD performed in a packed bed reactor under continuous flow confirmed the efficiency of the SiO2-βCD matrix in increasing the operational stability of system, revealing a half-life (t1/2 = 1040 h), 2.5 times greater than that achieved in the same system using lipase immobilized on SiO2.PVA (t1/2 = 430 h).
Keywords:Lipase;Hybrid matrices;Glycerol affinity;Lipase immobilization;Transesterification
  1. Sheldon RA, van Pelt S, Chem. Soc. Rev., 42, 6223 (2013)
  2. Dicosimo R, McAuliffe J, Poulose AJ, Bohlmann G, Chem. Soc. Rev., 42, 6437 (2013)
  3. Homaei A, Sariri R, Vianello F, Stevanato R, J. Chem. Biol., 6, 185 (2013)
  4. Liese A, Hilterhaus L, Chem. Soc. Rev., 42(15), 6236 (2013)
  5. Cazaban D, Wilson L, Betancor L, Curr. Org. Chem., 21(2), 96 (2016)
  6. Ansari SA, Husain Q, Biotechnol. Adv., 30, 512 (2012)
  7. Adlercreutz P, Chem. Soc. Rev., 42, 6406 (2013)
  8. Cipolatti EP, Manoel EA, Fernandez-Lafuente R, Freire DMG, Biotechnol. Res. Innov., 1, 26 (2017)
  9. Meunier SM, Kariminia HR, Legge RL, Production Technologies for Biofuels, John Wiley & Sons, New Jersey, p. 67 2017.
  10. Ding S, Cargill A, Medintz IL, Claussen JC, Curr. Opin. Biotechnol., 34, 242 (2015)
  11. Talbert JN, Goddard JM, Colloids Surf. B: Biointerfaces, 93, 8 (2012)
  12. Es I, Vieira JDG, Amaral AC, Appl. Microbiol. Biotechnol., 99(5), 2065 (2015)
  13. Barbosa O, Torres R, Ortiz C, Berenguer-Murcia A, Rodrigues RC, Fernandez-Lafuente R, Biomacromolecules, 14(8), 2433 (2013)
  14. Singh V, Srivastava P, Singh A, Singh D, Malviya T, Polym. Rev., 56, 113 (2016)
  15. Qi D, Cao Z, Ziener U, Adv. Colloid Interface Sci., 211, 47 (2014)
  16. Samuneva B, Kabaivanova L, Chernev G, Djambaski P, Kashchieva E, Emanuilova E, Salvado IMM, Fernandes MHV, Wu A, J. Sol-Gel Sci. Technol., 48, 73 (2008)
  17. Pandey S, Mishra SB, J. Sol-Gel Sci. Technol., 59, 73 (2011)
  18. Levy D, Zayat M, The Sol-gel Handbook: Synthesis, Characterisation and Applications, 3-vol. Set, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, 2015.
  19. Pingan H, Mengjun J, Yanyan Z, Ling H, RSC Adv., 7, 2450 (2017)
  20. Rangelova N, Aleksandrov L, Nenkova S, J. Sol Gel Sci. Technol. (2017), doi:http://dx.doi.org/10.1007/s1097.
  21. Todorova EV, Chernev GE, Djambazov SP, Open J. Inorg. Non Met. Mater., 4, 35 (2014)
  22. Cutrone G, Casas-Solvas JM, Vargas-Berenguel A, Int. J. Pharm., 531, 621 (2017)
  23. Xie K, Yu Y, Shi Y, Carbohydr. Polym., 78, 799 (2009)
  24. Da Ros PCM, Silva GAM, Mendes AA, Santos JC, de Castro HF, Bioresour. Technol., 101(14), 5508 (2010)
  25. Souza LTA, Mendes AA, De Castro HF, BioMed Res. Int., 13 (2016), doi:http://dx.doi.org/10.1155/2016/1404567 1404567.
  26. Rufino AR, Biaggio FC, Santos JC, de Castro HF, Int. J. Biol. Macromol., 47, 5 (2010)
  27. Santos JC, Paula AV , Nunes GFM, de Castro HF, J. Mol. Catal. B-Enzym., 52-53, 49 (2008)
  28. Ponyrko S, Kobera L, Brus J, Matejka L, Polymer, 54(23), 6271 (2013)
  29. Freitas L, Silva GS, Santos JC, Oliveira PC, de Castro HF, Eur. Food Res. Technol., 233, 743 (2011)
  30. Paula AV, Nunes GFM, de Castro HF, Santos JC, Ind. Eng. Chem. Res., 54(6), 1731 (2015)
  31. Carvalho AKF, Da Ros PCM, Freitas L, Andrade GSS, Zanin GM, De Castro HF, Ind. Crop. Prod., 50, 485 (2013)
  32. Silva WCe, Teixeira LF, Carvalho AKF, Mendes AA, de Castro HF, J. Ind. Eng. Chem., 20(3), 881 (2014)
  33. Simoes AS, Ramos L, Freitas L, Santos JC, Zanin GM, De Castro HF, Biofuel Res. J., 6, 242 (2015)
  34. Costa-Silva W, Freitas L, Oliveira PC, De Castro HF, Bioprocess. Biosyst. Eng., 39, 1611 (2016)
  35. Ramos L, Martin LS, Santos JC, de Castro HF, Ind. Eng. Chem. Res., 56(1), 1 (2017)
  36. Dossat V, Combes D, Marty A, Enzyme Microb. Technol., 25(3-5), 194 (1999)
  37. Tran DT, Chen CL, Chang JS, Appl. Energy, 168, 340 (2016)
  38. Severac E, Galy O, Turon F, Monsan P, Marty A, Bioresour. Technol., 102(8), 4954 (2011)
  39. Poppe JK, Fernandez-Lafuente R, Rodrigues RC, Ayub MAZ, Biotechnol. Adv., 33, 511 (2015)
  40. Hama S, Yoshida A, Tamadani N, Noda H, Kondo A, Bioresour. Technol., 135, 417 (2013)
  41. Azocar L, Navia R, Beroiz L, Jeison D, Ciudad G, New Biotechnol., 31, 422 (2014)
  42. Tacias-Pascacio VG, Virgen-Ortiz JJ, Jimenez-Perez M, Yates M, Torrestiana-Sanchez B, Rosales-Quintero A, Fernandez-Lafuente R, Fuel, 200, 1 (2017)
  43. Lima LN, Oliveira GC, Rojas MJ, de Castro HF, Da Ros PCM, Mendes AA, Giordano RLC, Tardioli PW, J. Ind. Microbiol. Biotechnol., 42, 523 (2015)
  44. Sasso F, Natalello A, Castoldi S, Lotti M, Santambrogio C, Grandori R, Biotechnol. J., 11, 954 (2016)
  45. Sanchez DA, Tonetto GM, Ferreira ML, Biotechnol. Bioeng., 115(1), 6 (2018)
  46. Da Ros PCM, Costa-Silva W, Grabauskas D, Perez VH, de Castro HF, Ind. Crop. Prod., 52, 313 (2014)
  47. Santos FD, Conceicao LRV, Ceron A, de Castro HF, Appl. Clay Sci., 149, 41 (2017)
  48. Duchene D, Bochot A, Int. J. Pharm., 514(1), 58 (2016)
  49. Varana G, Varana C, Erdogar N, Hıncalb AA, Bilensoy E, Int. J. Pharm., 531, 457 (2017)
  50. Fernandez-Lopez L, Pedrero SG, Lopez-Carrobles N, Virgen-Ortiz JJ, Gorines BC, Otero C, Fernandez-Lafuente R, Process Biochem., 54, 81 (2017)
  51. Palomo M, Munoz G, Fernandez-Lorente G, Mateo C, Fernandez-Lafuente R, Guisan JM, J. Mol. Catal. B-Enzym., 19-20, 279 (2002)
  52. Hernandez K, Garcia-Galan C, Fernandez-Lafuente R, Enzyme Microb. Technol., 49(1), 72 (2011)
  53. Rodrigues RC, Ortiz C, Berenguer-Murcia A, Torres R, Fernandez-Lafuente R, Chem. Soc. Rev., 42, 6290 (2013)
  54. Manoel EA, dos Santos JCS, Freire DMG, Rueda N, Fernandez-Lafuente R, Enzyme Microb. Technol., 71, 53 (2015)
  55. Vescovi V, Kopp W, Guisan JM, Giordano RLC, Mendes AA, Tardioli PW, Process Biochem., 51(12), 2055 (2016)
  56. Virgen-Ortiz JJ, Tacias-Pascacio VG, Hirata DB, Torrestiana-Sanchez B, Rosales-Quintero A, Fernandez-Lafuente R, Enzyme Microb. Technol., 96, 30 (2017)
  57. Zaak H, Kornecki JF, Siar EH, Fernandez-Lopez L, Corberan VC, Sassi M, Fernandez-Lafuente R, Process Biochem., 61, 95 (2017)
  58. Secundo F, Miehe-Brendle J, Chelaru C, Ferrandi EE, Dumitriu E, Microporous Mesoporous Mater., 109, 350 (2008)
  59. Mendes AA, de Castro HF, Andrade GSS, Tardioli PW, Giordano RDC, React. Funct. Polym., 73(1), 160 (2013)
  60. Zhao X, Qi F, Yuan C, Du W, Liu D, Renew. Sust. Energ. Rev., 44, 182 (2015)
  61. Szczesna-Antczak M, Kubiak A, Antczak T, Bielecki S, Renew. Energy, 34(5), 1185 (2009)