Malonic acid를 이용한 전처리가 꼬시레기의 가수분해에 미치는 영향

박미라; 정귀택; Mi-Ra Park; Gwi-Taek Jeong

Korean Chemical Engineering Research, Vol.56, No.4, 542-546, August, 2018

DOI10.9713/kcer.2018.56.4.542 Export Citation

Malonic acid를 이용한 전처리가 꼬시레기의 가수분해에 미치는 영향

Effect of Malonic Acid-Catalyzed Pretreatment on the Hydrolysis of Gracilaria verrucosa

박미라, 정귀택^†

부경대학교 생물공학과, 48513 부산광역시 남구 용소로 45

Mi-Ra Park, and Gwi-Taek Jeong^†

Department of Biotechnology, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513, Korea

E-mail:

초록

본 연구에서는 해양 거대조류 중 홍조류에 속하는 꼬시레기(Gracilaria verrucosa)로부터 malonic acid를 사용하여 열수 전처리 조건(전처리 온도, 촉매 농도, 고액비, 전처리 시간)에 따른 전처리와 효소가수분해를 통한 바이오슈거(환원당) 생산 가능성을 조사하였다. 300 mM malonic acid, 1:20 고액비 조건에서 130 °C에서 60분간 전처리를 수행하여 49.2%의 환원당 수율을 얻었다. 전처리 반응 후 이어진 효소 가수분해를 통하여 64.5%의 수율을 확보하였다.

In this study, the effects of malonic acid-catalyzed pretreatment on the subsequent enzymatic hydrolysis of red macro-algae Gracilaria verrucosa for production of biosugar (total reducing sugar) were investigated. In the hydrothermal pretreatment condition of 300 mM malonic acid, 1:20 solid-to-liquid ratio at 130 oC for 60 min, a 49.2% biosugar yield was achieved. Moreover, by subsequent enzymatic hydrolysis after pretreatment, maximum yield of 64.5% was achieved.

Keywords:Gracilaria verrucosa;Malonic acid-catalyzed pretreatment;Subsequent enzymatic hydrolysis;Biosugar;Total reducing sugar

[References]

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Odjadjare EC, Mutanda T, Olaniran AO, Critical Reviews Biotechnology, 37, 37 (2015)
Meinita MDN, Hong YK, Jeong GT, Bioprocess. Biosyst. Eng., 35, 123 (2012)
Ra CH, Choi JG, Kang CH, Sunwoo IY, Jeong GT, Kim SK, Micobiol. Biotechnol. Lett., 43, 9 (2015)
Kwon OM, Kim DH, Kim SK, Jeong GT, Algal Res., 13, 293 (2016)
Cho YK, Kim MJ, Kim SK, KSBB J., 28(6), 366 (2013)
Jeong GT, Park DH, Appl. Biochem. Biotechnol., 161(1-8), 41 (2010)
Jeong GT, Ra CH, Hong YK, Kim JK, Kong IS, Kim SK, Park DH, Bioprocess. Biosyst. Eng., 38, 207 (2015)
Kim DH, Lee SB, Jeong GT, Bioresour. Technol., 161, 348 (2014)
Ra CH, Kim YJ, Lee SY, Jeong GT, Kim CH, Kim YJ, Lee SY, Jeong GT, Kim SK, Bioprocess. Biosyst. Eng., 38, 1715 (2015)
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Jeong GT, Kim SK, Park DH, Bioresour. Technol., 181, 1 (2015)
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[1] Ra CH, Lee HJ, Shin MK, Kim SK, KSBB J., 28(5), 282 (2013)

[2] Jeong GT, Park DH, Korean Chem. Eng. Res., 53, 1 (2015)

[3] Kim AR, Kim DH, Jeong GT, KSBB J., 30, 53 (2015)

[4] Kim DH, Kim AR, Park DH, Jeong GT, Korean Chem. Eng. Res., 54(1), 70 (2016)

[5] Pierobon SC, Cheng X, Graham PJ, Nguyen B, Karakolis EG, Sinton D, Sustainable Energy Fuels, 2, 13 (2018)

[6] Odjadjare EC, Mutanda T, Olaniran AO, Critical Reviews Biotechnology, 37, 37 (2015)

[7] Meinita MDN, Hong YK, Jeong GT, Bioprocess. Biosyst. Eng., 35, 123 (2012)

[8] Ra CH, Choi JG, Kang CH, Sunwoo IY, Jeong GT, Kim SK, Micobiol. Biotechnol. Lett., 43, 9 (2015)

[9] Kwon OM, Kim DH, Kim SK, Jeong GT, Algal Res., 13, 293 (2016)

[10] Cho YK, Kim MJ, Kim SK, KSBB J., 28(6), 366 (2013)

[11] Jeong GT, Park DH, Appl. Biochem. Biotechnol., 161(1-8), 41 (2010)

[12] Jeong GT, Ra CH, Hong YK, Kim JK, Kong IS, Kim SK, Park DH, Bioprocess. Biosyst. Eng., 38, 207 (2015)

[13] Kim DH, Lee SB, Jeong GT, Bioresour. Technol., 161, 348 (2014)

[14] Ra CH, Kim YJ, Lee SY, Jeong GT, Kim CH, Kim YJ, Lee SY, Jeong GT, Kim SK, Bioprocess. Biosyst. Eng., 38, 1715 (2015)

[15] Ayeni AO, Omoleye JA, Mudliar S, Hymore FK, Pandey RA, Korean J. Chem. Eng., 31(7), 1180 (2014)

[16] American Chemical Society, https://www.acs.org/content/acs/en/molecule-ofthe-week/archive/m/malonic-acid.html, March 21, 2017.

[17] Hu HC, Liu YH, Li BL, Cui ZS, Zhang ZH, RSC Adv., 5, 7720 (2015)

[18] Mille GL, Anal. Chem., 31, 426 (1959)

[19] Jeong GT, Kim SK, Park DH, Bioresour. Technol., 181, 1 (2015)

[20] Tan IS, Lam MK, Lee KT, Carbohydr. Polym., 94, 561 (2013)