We report on the successful laser transfer of biocompatible composite coatings based on polyaniline (PANI) embedded with magnetite (PANI-Fe3O4) and Lincomycin hydrochloride (PANI-Lincomycin) or Lincomycin-functionalized magnetite (PANI-Fe3O4@Lincomycin) by matrix assisted pulsed laser evaporation (MAPLE) technique. The physico-chemical investigations revealed relevant data regarding the stoichiometric deposition, morphology and topography of the as-deposited coatings. Regarding the MAPLE coatings, the FTIR studies evidenced the vibrational bands characteristic to pristine PANI material, while the SEM investigations unveiled a preferential particulate morphology (with aggregates shape and size depending on the deposited material). Additionally, the AFM measurements indicated variations of RMS value, following the Lincomycin and magnetite incorporation. The wettability measurements displayed a hydrophilic behavior of the synthesized coatings, while the electrochemical studies emphasized an enhanced resistance against corrosion in simulated body fluid when compared with bare Ti. Cellular viability, immunofluorescence and SEM results proved that the MAPLE coatings were suitable materials for beneficial adhesion, spreading and proliferation of osteoblast-like cells (MG-63). Moreover, an increased efficiency was evidenced against Staphylococcus aureus biofilm development. The multifunctional properties of the laser processed composite coatings - confirmed by cumulative biocompatible, antimicrobial and anticorrosive behaviors - recommend them as promising solutions for biomedical applications.