Drying is an essential unit operation needed for safe storage and handling of the wet Distillers' spent grain (DSG), a major by-product of the ethanol industry. For the simulation and modeling of the drying process, a detailed study on different pre-requisite parameters such as the thermo-physical properties and effective moisture diffusivity of the material to be dried is required. The present study reports the effective moisture diffusivity and activation energy of the DSG pellets during superheated steam (SS) drying. Cylindrical DSG pellets at two moisture mass fractions (25 and 35%) and three mass fractions of distillers' solubles (0,10, and 30%) were dried at three SS temperatures (120, 135, 150, 165, and 180 degrees C) and three SS velocities (0.5, 1.0, and 1.5 m/s), respectively. The experiment-based moisture diffusivity of the DSG pellet with and without solubles was determined by using the drying characteristic and the analytical solution of Fick's law of diffusion. The results showed that the effective diffusivity increased with an increase in SS temperature and velocity and its value was in the range of 2.49 x 10(-9) to 17.9 x 10(-9)m(2)/s. The dependency of the moisture diffusivity on temperature and moisture mass fraction was established by using Arrhenius equation and Levenberg-Marquardt optimization algorithm. The model coefficient and constants were compared with the calculated values of instantaneous effective moisture diffusivity with the mean relative percentage deviation <= 10%. These findings could serve as a fundamental input for the numerical modeling of SS drying of DSG.