The present work explores the heat transfer, pressure loss and thermal enhancement factor (TEF) behaviors of a tube fitted with regularly spaced quadruple twisted tape elements (RS-QTTs). The effects of free space ratio and twisted tape arrangement (co, counter and cross arrangements) were investigated in turbulent flow regime. Experiments were carried out using all twisted tapes with constant twist ratio of 2.5 under constant heat flux condition. The results show that RS-QTTs in all arrangements yield lower heat transfer rate and cause lower friction loss than full-length quadruple twisted tapes (QTTs) due to the weaker swirling intensity caused by the decaying of swirling flow. Heat transfer rates given by the RS-QTTs with s/y of 0.5, 1.0, 1.5 and 2.0 are lower than those given by the QTTs by around 6.2%, 8.13%, 10.6% and 12.8%, respectively. For RS-QTTs, heat transfer rate, friction loss and TEF increase with decreasing s/y. Over the range reported, the RS-QTTs in cross arrangement with s/y = 0.5 gives heat transfer rate up to 6.6% over than that of the QTTs and the maximum TEF of 1.27. Moreover, the flow and thermal behaviors were also numerically studied for a better understanding on heat transfer mechanism.