A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14(T) was isolated from mangrove soil samples collected on chengmai, Hainan province, China. Strain Q14(T) was able to grow at 10-40 degrees C (optimum 30 degrees C), pH 5.5-10.0 (optimum 6.5-8.0) and with 0.5-6% (w/v) NaCl (optimum 1%). The genomic DNA G+C content was 70.1%. The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C-15:0, iso-C-17:0 and anteiso-C-17:0. The major polar lipids of strain Q14(T) were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid. The strain Q14(T) contained 2,4-diaminobutylic acid (A(2)bu), alanine acid, glutamic acid and glycine in the peptidoglycans. The phylogenetic analysis and DNA-DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14(T) as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp. nov. is proposed. The type strain is Q14(T) (= MCCC 1K03191(T) = KCTC 39814(T)).