Emulsifiers like polysorbate family, carboxymethyl cellulose are widely used in the preparation of drugs, vaccines, food, cosmetics, and skin care products. In this study, strain SP2 is isolated for the high-efficiency utilization of emulsifier as its sole carbon and energy source. Strain grows on the polysorbate family with generation time ranging from 0.8 to 2.2 h. It is sensitive to ampicillin, gentamicin, imipenem, penicillin, and vancomycin, but resistant to amikacin, cefoxitin, erythromycin, kanamycin, nalidixic acid, and tetracycline. Based on 16S rRNA gene, strain is identified as Acinetobacter soli. Compared to M. pamicella, which is capable of degrading 50% polysorbate 80 within 3 days, isolate PS2 could dispose 51.6% polysorbate 80 within only 8 h. Using cell crude extract from strain SP2, the liberation of free fatty acid in the reaction mixture containing polysorbate 80 suggests that the mechanism for polysorbate utilization belongs to the beta-oxidation.