The application of a new biochemical combustion technology, using a nanoenzyme (3EC) product, was studied in a 450 MW power plant. The enzyme changes the structure of the coal during the treatment process. The boiler performance and amount of air pollution were measured and analyzed. There was a clear decrease in the slagging observed on the heat-exchanger tubes. This is attributed to the formation of a softer, more porous iron-containing aluminosilicate slag structure. As a result, the slag does not cling to the heat-exchange tubes as tightly. Burning the enzyme-treated coal resulted in a decrease in NOx emissions by 16.72-21.43% during the entire test period and a decrease in SO2 emissions by 5.42-7.31% during the 85% load condition compared to emissions from the raw coal tests. In addition, sub-micrometer particles and mercury distribution were also influenced by the enzyme.