Surfactant dispersants have been introduced as a proper candidate to mitigate the problems caused by asphaltene precipitation, such as clogging wells, flowlines, and surface facilities in oil industry. In this work, we study the effects of dodecylbenzenesulfonic acid (DBSA) as a surfactant on asphaltene deposition on stainless-steel and glass surfaces. Experiments were conducted to measure asphaltene precipitation in the bulk system and asphaltene deposition on the stainless-steel and glass surfaces. Results revealed that the surfactant delays the asphaltene onset in the bulk system. However, asphaltene deposition on the stainless-steel surface was increased at all measured concentrations of the surfactant, while the deposition rate on the glass surface decreased by increasing the surfactant concentration. Affinity of the surfactant molecules to the stainless-steel surface was verified in asphaltene deposition and removal tests. The results revealed that the DBSA surfactant is able to remove deposited asphaltene on glass surfaces at high concentrations. This study reveals the importance of surface properties when the surfactant is used as an asphaltene dispersant.