The aim of this study was to focus on the optimization of various process parameters such as time (days), pH, and electrode type on electricity production by a microbial fuel cell (MFC). The efficiency of MFC was examined based on the current (A) and potential (V) measurements. In MFC, the anode section was filled with 500 mL of rumen fluid, slaughter house waste, and 2 g of hay as substrate. The cathode section was filled with distilled water, which acted as the air cathode. The results obtained confirmed that copper anode explores the maximum efficiency compared to stainless steel and aluminum. The biofilm attached to the electrode is electrochemically active as per the redox potential shown in cyclic voltammogram results.