In this work, metal-organic framework compound CPL-1 of a formula {[Cu-2(pzdc)(2)(pyz)]center dot 2H(2)O)(n) (pzdc = pyrazine-2,3-dicarboxylate, pyz = pyrazine) possessing kinetic quantum sieving effect on H-2/D-2 separation was selected to prepare two new chromatography stationary phase materials CPL-1@gamma-Al2O3 and MnCl2@CPL-1@gamma-Al2O3 by loading CPL-1 on gamma-Al2O3 particles with size 80-100 mesh through repeating in situ crystallization process, and afterwards by loading MnCl2 on CPL-1@gamma-Al2O3 via impregnation approach. The two optimized materials were thoroughly characterized by IR, XRD, SEM, EDS and TG/DTA method. The experimental results showed that, under liquid nitrogen temperature by using just 1-m chromatographic column and cheap He gas as carrier gas, the MnCl2@CPL-1@gamma-Al2O3 can solve the problem of long separation time and trailing phenomenon existing in the CPL-1@gamma-Al2O3 column, can realize highly effective hydrogen isotope H-2/D-2 separation with good separation resolution (R = 1.62) and approximate Gauss distribution of chromatographic peak, can be used for both qualitative and quantitative analysis of hydrogen isotope H-2/D-2 with short separation time of 6.5 min. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.