AimsOleaginous yeasts were isolated from five different rotten fruits and their oleaginous behaviour was characterized with varying carbon source and concentration (C/N) to identify their possible future applicabilities. Methods and ResultsSixteen yeasts were isolated, those accumulating around 20% of their cell dry weights as lipids were screened and identified as Candida tropicalis and Pichia kudriavzevii. Among glucose, maltose and sucrose, glucose was found favourable carbon source in terms of biomass yield and lipid content. Carbon concentration corresponding to C/N ratio 100/2 (C. tropicalis) and 120/2 (P. kudriavzevii) exhibited highest lipid contents812 and 757%and lipid yields11 and 3gl(-1), respectively. Lipids contained 16-28 types of triacylglycerols, and major fatty acids detected were caprylic, caparate, lauric, stearic palmitic, oleic, linoleic, aracidic and erucic acid with caprylic acid as predominant one. Fatty acid profile also witnessed variations with changing C/N. ConclusionsNoteworthy lipid contents were achieved in the two isolates higher than any existing reports on them. Pichia kudriavzevii exhibited reasonable overall lipid yield extending the opportunity of improvement upon changing the cultivation conditions. The lipids contained a range of fatty acids with a predominance of caprylic acid having unequivocal biotechnological importance. Significance and Impact of the StudyTwo potent oleaginous yeast isolates were studied, and this is the only successive report describing oleaginous behaviour of P. kudriavzevii. The cultivation parameters such as C source, concentration and C/N ratio which critically influence the oleaginous behaviour, lipid content, yield and composition have been accessed so as to provide comprehensive understanding on single cell oil production from these isolates. Study progressively contributes to current and upcoming researches in microbial oils and the characteristic fatty acid, and TAG profile provides important leads for their putative applicabilities.