Hypothesis: Electrochemical impedance spectroscopy is useful to monitor anionic interactions with a Lipid Cubic Phase, as previously demonstrated for cationic interaction (Khani Meynaq et al., 2016). It was expected that the smaller hydrophilic anions, acetate and chloride, would interact differently than the large tryptophan anion with its hydrophobic tail. Experiment: The impedance measurements enabled estimation of resistances and capacitances of a freestanding lipid cubic phase membrane at exposure to 4 and 40 mM solutions of NaCI, Na0Ac and NaTrp. Small-angle X-ray scattering was used for cubic phase identification and to track structural changes within the cubic phase when exposed to the different electrolytes. Findings: The membrane resistance increases at exposure to the electrolytes in the order Cl- < OAc- < Trp(-). The membrane resistance decreases with time at exposure to the hydrophilic anions and increases with time at Trp(-) exposure. The membrane capacitances were lower for NaTrp compared to NaCI and NaOAc at the corresponding concentrations which is consistent with the results from SAXRD. It is concluded that Trp(-) ions do not enter the aqueous channels of the cubic phase but are strongly adsorbed to the membrane/electrolyte interface leading to large alteration of the lipid phase structure and a high membrane resistance. (C) 2018 Elsevier Inc. All rights reserved.