In this work, a new electrochemical sensor was fabricated based on Sr2PdO3 nano-perovskite manually mixed with carbon nanotubes (CNTs) and tasted on glassy carbon (GC) electrode surface; GC/(CNTs-Sr2PdO3). Enhanced sensitivity, selectivity, stability and electron transfer kinetics were realized by the application of nano-perovskite modified carbon nanotubes composite for determination of dobutamine (DB). The proposed sensor showed high electro-catalytic activity in the linear dynamic range of 0.03 mu mol L-1 to 50 mu mol L-1 with sensitivity of 20.91 mu A/mu mol L-1, detection limit of 0.012 nmol L-1 and a quantification limit of 0.04 nmol L-1. The proposed method was successfully applied for DB detection in blood serum samples with good recovery results. The sensor showed anti-interfering ability in presence of common interferences as ascorbic acid, uric acid, acetaminophen, serotonin and morphine.