A new, highly selective, sensitive and stable enzymatic glucose sensor was fabricated on glassy carbon electrode (GCE) using zinc oxide (ZnO) nanoparticles embedded nitrogen-doped carbon sheets (ZnO@NDCS), glucose oxidase (GOx) (assigned as GCE/ZnO@NDCS/GOx). First, ZnO@NDCS were synthesized by a simple hydro thermal method. Zn powder, aqueous ammonia, and peach extract were served as the precursor for ZnO NPs, nitrogen and carbon, respectively. The fabricated GCE/ZnO@NDCS/GOx biosensor exhibited a high and reproducible sensitivity of 231.7 mu A mM(-1) cm(-2). Also, showed a wide linear range from 0.2 to 12 mM with a correlation coefficient R-2 = 0.998 and lowest detection limit (based on S/N ratio = 3) of 6.3 mu M. The GCE/ ZnO@NDCS/GOx biosensor is acceptably stable, selective, and it was successfully applied to the quantitative monitoring of glucose in human blood serum. The synthesized ZnO@NDCS nanocomposite may be found useful in other applications in the fields of solar cells and optoelectronic devices. These encouraging results suggest a simple and effective method obtain electrode material for the enzymatic glucose sensor.