Every kilogram of the harvested lemon basil (Ocimum citriodorum Vis.) seed generates 1-1.5 kg of basil straw, which is commonly left in the field or burned in the open field. To valorize this straw, the production of caryophyllene oxide from basil straw by hydrodistillation, n -hexane, and supercritical CO2 (SCCO2) extraction was studied. Caryophyllene oxide offers significant anticancer properties. The maximum extracted yields from basil straw by hydrodistillation, n -hexane, and SCCO2 extraction were 0.07% 0.01%, 4.11% 0.25%, and 1.21% +/- 0.09% (w/w), respectively. The CO2 consumption of crude extracted was 16.47 kg-CO2/g extracts at 60 degrees C and 30.0 MPa. Other bioactive compounds, such as linalool, neral, and geranial are co-extracted.