Uvaia (Eugenia pyriformis Cambess.) leaves were subjected to extraction with supercritical fluid extraction (SFE) and ultrasound-assisted extraction (UAE) under different conditions and the results were compared with a conventional maceration extraction. The extracts obtained with SFE were chemically characterized by gas chromatography-mass spectrometry (GC-MS), which showed that beta-amyrin (53.72%) and a-amyrin (24.63%) were their main compounds. The best results for SFE and UAE yieldings were 1.69% and 1.79%, respectively. The best results for amyrin content, by extract weight, were 81.19% for SFE and 77.03% for UAE. The highest amyrin content was 11.40 g of the isomers mixture per kg of dry leaf in SFE and 11.46 g kg(-1) in UAE. Scanning electron microscopy analysis showed differences in leaf particles before and after the extraction process, caused by the removal of compounds by the extraction techniques studied.