The polymorphism of phospholipid mono layers has been extensively studied because of its importance in surface thermodynamics, soft matter physics, and biomembranes. To date, the phase behavior of phospholipid monolayers has been nearly exclusively studied with the classical Langmuir-type film balance. However, because of experimental artifacts caused by film leakage, the Langmuir balance fails to study the reversibility of two-dimensional surface phase transitions. We have developed a novel experimental methodology called the constrained drop surfactometry capable of providing a leakage-proof environment, thus allowing reversibility studies of two-dimensional surface phase transitions. Using dipalmitoylphosphatidylcholine (DPPC) as a model system, we have studied the reversibility of isothermal and isobaric phase transitions in the monolayer. It is found that not only the compression and expansion isotherms but also the heating and cooling isobars, completely superimpose with each other without hysteresis. Microscopic lateral structures of the DPPC monolayer also show reversibility not only during the isothermal compression and expansion processes but also during the isobaric heating and cooling processes. It is concluded that the two-dimensional surface phase transitions in phospholipid monolayers are reversible, which is consistent with the reversibility of phase transitions in bulk pure substances. Our results provide a better understanding of surface thermodynamics, phase change materials, and biophysical studies of membranes and pulmonary surfactants.