Hydrophilic gigaporous polystyrene (HGPS) microspheres with bimodal pore distributions were first synthesized" I), using amphiphilic diblock glycopolymer (ADD) and toluene as macroporogen and mesoporogen, respectively. The effects of different HLB values, molecular weights, and concentrations of ADG as well as the addition of mesoporogen on the morophology of microspheres were investigated in detail. The HGPS microspheres were characterized to possess two type of pores, i.e gigapores (300-7000 nm) and mesopores (4-50 nm), As a consequence, the column packed with HGPS migrospheres presented low backpressure, good permeability and high column efficiericy, indicating the existence of flow through pores in the particle. Compared to native polystyrene microsheres, the nonspecific adsorption of proteins on HGPS microspheres was greatly reduced by 93.2%. The HGPS microspheres can be easily derivatized by classical methods since their surface was hydroxyl-rich glycopolmers. All the results indicate that the HGPS microspheres have great potentials in high-speed protein chromatography, cell culture, and enzpme immobilization.