Star-shaped self-assembled micelles of block copolymer chitosan-co-poly(ethylene glycol) methyl ether methacrylate hydrogel were successfully synthesized by free-radical copolymerization and were investigated against Fluorescein as an anticancer hydrophobic model drug. The chemical structures of synthesized hydrogel were characterized by FT-IR, TGA, DSC, FESEM, AFM, and their interfaces in water, Fluorescein, salt, and urea were studied using dynamic light scattering (DLS) technique. The self-assembled blank and fluorescein-loaded micelles with an average size (z average ae 50-350 nm), critical micelle concentration (CMC approximate to 2.90 mg L-1), maximum stability (zeta potential approximate to 30 mV), electrophoric mobility approximate to 22 mV, conductivity approximate to 250 mV, and lower critical solution temperature (LCST ae 38.80 degrees C) were explored. The in vitro studies revealed 78% loading and 66% release profiles. The DLS and drug administration profiles suggested the system as hydrophobic anticancer drug carrier at physiological conditions.