A batch of new cation-exchange membranes (CEM(n)) and three batches of used cation-exchange membranes (CEM(u)) at various use durations, from different electrodialysis (ED) units used in food industry, were characterized to evaluate the effects of prolonged use and organic fouling on their microstructure. Different characterization methods were experimented: ion-exchange capacity (IEC), thickness (T-m), conductivity (k(m)), contact angle (theta), water content (W-c), volume fraction of the inter-gel solution (f(2)), tensile strength tests, FTIR, structural and elementary SEM analysis and morphological analysis by optical microscopy. Non-aggressive, easy and economic cleaning strategy was tested in the ex-situ static mode. Three solutions (35 g L-1 NaCl, reconstituted seawater and a water-ethanol mixture) were used as regenerative solutions, where the membranes were soaked from 2 to 120 h. The efficiency of the cleaning procedures were evaluated, for each sample of treated membrane, by following km, IEC, theta, and f(2) as functions of the cleaning time in order to judge the recovery of their physicochemical properties. The water-ethanol cleaning method was found to be the most efficient for these membranes. This method leads, first, to significant increases in km, IEC and 12 (at least 47%, 26% and 63%, respectively after 120 h) which indicates the effectiveness of internal cleaning, and second, to a decrease of about 15% in theta (after 120 h), that confirms the stripping of a part of the external fouling layer. We have used the microheterogeneous model to demonstrate the progressive increase of f(2) as a function of the cleaning duration with the water-ethanol mixture.