The present study is conducted to propose a new code for optimizing a multicomponent isotope separation cascade. An efficient code is developed using an enhanced TLBO algorithm with a novel mutation for calculating the optimal parameters. The results indicate that by implementing a new objective function derived from the combination of D function with total interstage flow rates, the optimum tapered cascade is more efficient than the optimal square one. Also, the best feed locations for the separation of the middle components in the square cascade are the middle stages.