PEGylated Gold Nanoprobe Bearing the Diselenide Bond for ROS-Responsive Fluorescence Imaging

Veerasikku Gopal Deepagan; E. K. Pramod Kumar; Yung Doug Suh; Jae Hyung Park

Macromolecular Research, Vol.26, No.7, 577-580, July, 2018

DOI10.1007/s13233-018-6085-1 Export Citation

PEGylated Gold Nanoprobe Bearing the Diselenide Bond for ROS-Responsive Fluorescence Imaging

Veerasikku Gopal Deepagan¹, E. K. Pramod Kumar¹, Yung Doug Suh^{1, 2}, and Jae Hyung Park^{1, 3, †}

¹School of Chemical Engineering, College of Engineering, Sungkyunkwan University, Suwon, Gyeonggi 16419, Korea
²Research Center for Convergence Nanobiotechnology (RC2NT), Korea Research Institute of Chemical Technology, Daejeon 34114, Korea
³Biomedical Institute for Convergence at SKKU, Sungkyunkwan University, Suwon, Gyeonggi 16419, Korea

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Reactive oxygen species (ROS)-sensitive imaging can be used to distinguish ROS-rich diseased tissues from normal tissues. Herein, we developed a selective H2O2 concentration-dependent on-off gold nanoprobe and demonstrated its in vitro fluorescence imaging application using activated macrophages cells. The nanoprobe was prepared by covalent conjugation of polyethylene glycol and fluorescein dyes on gold nanoparticle surface through a H2O2-sensitive diselenide linker. The nanoprobes were well-dispersed in aqueous solution and inactive in H2O2-deficient conditions, ascribed to fluorescence resonance energy transfer between dye and gold nanoparticles. Once the nanoprobe was exposed to an H2O2-rich environment, it was quickly activated by diselenide bond dissociation, leading to fluorophore release from its surfaces. Overall, gold nanoprobe with the diselenide linker might be a potential candidate for ROS imaging.

Keywords:gold nanoprobe;diselenide linker;polyethylene glycol;H2O2;ROS imaging

[References]

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Deepagan VG, You DG, Um W, Ko H, Kwon S, Choi KY, Yi GR, Lee JY, Lee DS, Kim K, Nano Lett., 16, 6257 (2016)
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Lee ES, Deepagan V, You DG, Jeon J, Yi GR, Lee JY, Lee DS, Suh YD, Park JH, Chem. Commun., 52, 4132 (2016)
Dulkeith E, Ringler M, Klar TA, Feldmann J, Javier AM, Parak WJ, Nano Lett., 5, 585 (2005)
Powell RD, Halsey CM, Hainfeld JF, Microsc. Res. Tech., 42, 2 (1998)
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Schneider G, Decher G, Nerambourg N, Praho R, Werts MHV, Blanchard-Desce M, Nano Lett., 6, 530 (2006)
Deepagan VG, Kwon S, You DG, Um W, Ko H, Lee H, Jo DG, Kang YM, Park JH, Biomaterials, 103, 56 (2016)
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[Referenced By]

[1] Valenti VE, de Abreu LC, Ferreira C, Saldiva PH, Oxidative Stress Diseases, 3, 41 (2012)

[2] Alfadda AA, Sallam RM, J. Biomed. Biotechnol., 2012, 1 (2012)

[3] Houstis N, Rosen ED, Lander ES, Nature, 440, 944 (2006)

[4] Deepagan VG, You DG, Um W, Ko H, Kwon S, Choi KY, Yi GR, Lee JY, Lee DS, Kim K, Nano Lett., 16, 6257 (2016)

[5] Liou GY, Storz P, Free Radical Res., 44, 479 (2010)

[6] Bienert GP, Schjoerring JK, Jahn TP, Biochim. Biophys. Acta, 1758, 994 (2006)

[7] Murphy MP, Biochem. J., 417, 1 (2009)

[8] Holmstrom KM, Finkel T, Nat. Rev. Mol. Cell Biol., 15, 411 (2014)

[9] Papa S, Skulachev V, Detection of Mitochondrial Diseases, Springer, 174, 305 (1997).

[10] Lee SH, Gupta MK, Bang JB, Bae H, Sung HJ, Adv. Healthc. Mater., 2, 908 (2013)

[11] Trachootham D, Alexandre J, Huang P, Nat. Rev. Drug Discov., 8, 579 (2009)

[12] Uusitalo LM, Hempel N, Int. J. Mol. Sci., 13(9), 10660 (2012)

[13] Lee ES, Deepagan V, You DG, Jeon J, Yi GR, Lee JY, Lee DS, Suh YD, Park JH, Chem. Commun., 52, 4132 (2016)

[14] Dulkeith E, Ringler M, Klar TA, Feldmann J, Javier AM, Parak WJ, Nano Lett., 5, 585 (2005)

[15] Powell RD, Halsey CM, Hainfeld JF, Microsc. Res. Tech., 42, 2 (1998)

[16] Dubertret B, Calame M, Libchaber AJ, Nat. Biotechnol., 19, 365 (2001)

[17] Alkilany AM, Murphy CJ, J. Nanopart. Res., 12, 2313 (2010)

[18] Schneider G, Decher G, Nerambourg N, Praho R, Werts MHV, Blanchard-Desce M, Nano Lett., 6, 530 (2006)

[19] Deepagan VG, Kwon S, You DG, Um W, Ko H, Lee H, Jo DG, Kang YM, Park JH, Biomaterials, 103, 56 (2016)

[20] Han P, Ma N, Ren HF, Xu HP, Li ZB, Wang ZQ, Zhang X, Langmuir, 26(18), 14414 (2010)

[21] Ma N, Li Y, Ren H, Xu H, Li Z, Zhang X, Polym. Chem., 1, 1609 (2010)

[22] Saravanakumar G, Kim J, Kim WJ, Adv. Sci., 4, 160012 (2017)

[23] Matsumura Y, Maeda H, Cancer Res., 46, 6387 (1986)