화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.67, 80-91, November, 2018
DOI10.1016/j.jiec.2018.06.017  Export Citation
Agglomeration of human dermal fibroblasts with ECM mimicking nano-fragments and their effects on proliferation and cell/ECM interactions
Taufiq Ahmad1, 2, Young Min Shin1, Jinkyu Lee1, 2, Hyeok Jun Shin1, 2, Sajeesh Kumar Madhurakart Perikamana1, 2, and Heungsoo Shin1, 2, †
  • 1Department of Bioengineering, Hanyang University, Seoul 133-791, Republic of Korea
  • 2BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Republic of Korea
E-mail:
Here, we engineered spheroids by using ECM mimicking nano-fragments (NFs) with fibroblasts and investigated their effect on proliferation and cell/ECM interactions. NF incorporation resulted in formation of a stable spheroid, which improved proliferation and viability of cells by assisting oxygen transport confirmed by LOX-1 staining. In addition, hypoxic and apoptotic genes were significantly downregulated in spheroids with PD-NFs. Furthermore, ECM and cell junction proteins were highly expressed. Overall, our findings suggest that incorporation of NFs within spheroids for assembly with various cell types can be an alternative approach for 3D cell culture in many applications.
Keywords:Nano-fragment (NF);NF-spheroids;Hybrid spheroids;Composite spheroid;3D culture;Polydopamine;ECM
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