알지네이트/수용성 프러시안 블루/환원된 그래핀 옥사이드 복합체를 이용한 전극 개질에 따른 과산화 수소 센서 개발

윤옥자; Ok Ja Yoon

Polymer(Korea), Vol.42, No.6, 1104-1109, November, 2018

DOI10.7317/pk.2018.42.6.1104 Export Citation

알지네이트/수용성 프러시안 블루/환원된 그래핀 옥사이드 복합체를 이용한 전극 개질에 따른 과산화 수소 센서 개발

A Hydrogen Peroxide Electrochemical Sensor Based on an Alginate/Water-Soluble Prussian Blue/Reduced Graphene Oxide Composite Modified Electrode

윤옥자^†

중앙대학교 다빈치 교양대학

Ok Ja Yoon^†

Da Vinci College of General Education, Chung-Ang University, Seoul 09974, Korea

E-mail:

초록

다양한 바이오 응용에서 중요한 측정 인자인 과산화 수소(H2O2) 센서를 개발하기 위하여, 알지네이트(A) 용액에 수용성 프러시안 블루(s-PB), 그리고 환원된 그래핀 옥사이드(rGO)를 분산시켜 전극 소재를 만들었고 탄소 스크린 프린트 전극에 복합 용액을 젤화 과정을 통해 전극에 고정시켰다. 젤화 과정에서 전극 소재에 남아 있는 염을 제거하기 위하여 충분히 수세를 한 후 실온에서 건조하였다. 전극 소재의 특성은 푸리에 변환 적외선 (FTIR), 전계방출 주사 전자 현미경(FE-SEM)으로 조사하였다. A/s-PB/rGO 전극을 이용한 순환 전류(CV) 측정 결과를 통해 전극으로써 우수한 전기 화학 반응, 안정성, 재현성을 확인하였고, 미분 펄스 볼타 그램(DPV)으로 측정한 산화 전류는 4 mM(S/N = 3)의 검출 한계와 높은 감도(2.7 μA · mM-1· cm-2)를 나타내었다. 전기화학적 센서 기반의 전극 개발은 의학 및 환경 응용 분야에서 매우 중요하게 활용될 것으로 판단된다.

An electrochemical H2O2 sensor was developed using an alginate/water-soluble prussian blue/reduced graphene oxide (A/s-PB/rGO) composite on carbon screen printed electrodes for use in bioapplications. Carbon screenprinted working electrodes were modified with A/s-PB, A/rGO, and A/s-PB/rGO composite solutions by in situ gelation. The composites were characterized by Fourier transform infrared spectroscopy and field emission scanning electron microscopy. The cyclic voltammetric measurements made using the electrode modified by A/s-PB/rGOs showed improved electrochemical reaction and stability over the A/s-PB and A/rGO modified electrodes. A two-electron transfer process is indicated at the modified electrode surface. The oxidation current showed a detection limit of 4 mM (S/N = 3) and linear range of 4.28 mM. The A/s-PB/rGO modified electrode provides a tool for high-sensitivity (2.7 μA·mM-1·cm-2) and stable H2O2 analyses with a low limit of detection. Development of biosensor-based modified electrodes is important for biomedical and environmental applications.

Keywords:A/s-PB/rGO modified electrode;A/s-PB modified electrode;A/rGO modified electrode;electrochemical sensor;hydrogen peroxide

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[9] Kuila T, Bose S, Khanra P, Mishra AK, Kim NH, Lee JH, Biosens. Bioelectron., 26, 4637 (2011)

[10] Shao Y, Wang J, Wu H, Liu J, Aksay IA, Lina Y, Electroanalysis, 22, 1027 (2010)

[11] Wei M, Qiao L, Zhang HG, Karakalos S, Ma K, Fu Z, Swihart MT, Wu G, Electrochim. Acta, 258, 735 (2017)

[12] Wei J, Qiu J, Li L, Ren L, Zhang X, Chaudhuri J, Wang S, Nanotechnology, 23, 335707 (2012)

[13] Wang YL, Zhao GC, Int. J. Electrochem., 6, 2011 (2011)

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[15] Bai XY, Chen GH, Shiu KK, Electrochim. Acta, 89, 454 (2013)

[16] Li J, Jiang Y, Zhai Y, Liu H, Li L, Anal. Lett., 48, 2786 (2015)

[17] Zhou M, Zhai YM, Dong SJ, Anal. Chem., 81, 5603 (2009)

[18] Velevska J, Pecovska-Gjorgjevich M, Stojanov N, Najdoski M, IJSBAR, 25, 380 (2016)

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[20] Karyakin AA, Electroanalysis, 13, 813 (2001)

[21] Krap CP, Balmaseda J, Zamora B, Reguera E, Int. J. Hydrog. Energy, 35(19), 10381 (2010)

[22] Schmidtke-Schrezenmeier G, Urban M, Musyanovych A, Milander V, Rojewski M, Fekete N, Menard C, Deak E, Tarte K, Rasche V, Landfester K, Schrezenmeier H, Cytotherapy, 13, 962 (2011)

[23] Liu D, Chen C, Hu G, Mei Q, Qiu H, Long G, Hu G, Acta Biochim. Biophys. Sin., 43, 301 (2011)

[24] Wei B, Wang J, Chen Z, Chen G, Chem. Eur. J., 14, 9779 (2008)

[25] Feng JJ, Ding H, Yang G, Wang RT, Li SG, Liao JN, Li ZY, Chen DM, J. Colloid Interface Sci., 508, 387 (2017)

[26] Basu H, Saha S, Pimple MV, Singh RK, J. Environ. Chem. Eng., 6, 4399 (2018)

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[28] Yoon OJ, Kim CH, Sohn IY, Lee NE, Sens. Actuators B-Chem., 88, 454 (2013)

[29] Mazloum-Ardakani M, Rajabi H, Bietollahi H, J. Argent. Chem. Soc., 97, 106 (2009)

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[32] Cinti S, Arduini F, Moscone D, Palleschi G, Killard AJ, Sensors, 14, 14222 (2014)