The aim of this study was to investigate the characteristics and the defoamer capacity of bio-oils obtained from textile sludge pyrolysis. The pyrolysis was carried out at two temperatures: 310 and 500 degrees C, and the bio-oils were analyzed after 7 days and after 2 months of storage under refrigeration. The structure of the bio-oils was determined by Fourier transform infrared spectroscopy (FTIR) and the extraction of polar compounds by solid phase micro extraction (SPME) coupled with gas chromatography/mass spectrometry (GC/MS) analysis. According to the chromatography and FTIR results, aromatic hydrocarbons, amines, silicone, and organic sulphur compounds were present in the pyrolysis oils. The Bikerman test showed that 1 mL of bio-oil obtained from pyrolysis at 500 degrees C can break down a column of foam in less than 1 min, which is comparable to the results of commercial antifoams. According to these results, these oils can be used as defoamers, even at the textile plant itself.