A free-standing palladium/reduced graphene oxide paper (Pd/rGOP) based on one-pot co-reduction was prepared. The as-prepared Pd/rGOPs contain evenly distributed Pd particles interposing between the rGOP layers. The structure and composition of Pd/rGOP were characterized by SEM, EDX, XRD and Raman spectra. We investigated the glucose sensing performance of Pd/rGOP by chronoamperometry, the sensitivity reach as high as 6.70 mu A mM(-1) cm(-2) with the linear range between 0.5 mM and 8 mM. The sensor also exhibits a good anti-interference ability and detection selectivity toward the interference. The proposed sensor has great potential to become a reliable tool in point-of-care medical devices.