Digital tongue images are usually acquired by a camera under specific illumination environments. In order to guarantee better color representation of the tongue body, we propose a novel tongue Color Rendition Chart acting as a color reference to be used in color calibration algorithms to standardize the captured tongue images. First, based on a large tongue image database captured with our digital tongue image acquisition system, we establish a statistical tongue color gamut. Then, from the first step, different quantities of colors in the Color Rendition Chart are determined via experimentation. Afterwards, results using X-Rite's ColorChecker (R) Color Rendition Chart (a standard in the color calibration field) are compared with the proposed tongue Color Rendition Chart by applying the color difference calculation formula of CIELAB and CIEDE2000 as a reference for the mean color calibration error. The results show that the proposed tongue Color Rendition Chart, which has 24 colors, produces a much smaller error (CIELAB 8.0755/CIEDE 20006.3482) compared with X-Rite's ColorChecker (R) Color Rendition Chart (CIELAB 197614.7836/CIEDE 200011.7686). This demonstrates the effectiveness of the novel tongue Color Rendition Chart.