Anaerobic Digestion (AD) is a promising technology to treat slaughterhouse waste (SHW) for the bioenergy production, nutrient recovery, and pathogen reduction. Three scenarios of estimating the biomethane potential from SHW of the US and the individual States have been conducted in this study. The US meat producers discharged 18.4 million metric ton (MT) SHW in 2016, which can potentially generate 22.7 x 10(9) kWh of biomethane. In Texas State, up to 2.3 x 10(9) kWh year(-1) of energy can be potentially produced from AD of SHW and up to 106.3% of natural gas currently used in the Nebraska State for electricity generation can potentially be replaced with biomethane produced from the AD of its SHW. The technical challenges using SHW as the feedstock of AD process in the US have been summarized, including nitrogen and sulfur inhibition, foaming, sterilization, digestate disposal, supply chain etc. In addition, case studies were conducted by proposing an AD plant treating SHW from a typical slaughterhouse in a U.S. State that mostly processes the broiler, swine, and bovine. In our case studies, a net energy of 17,009-85,278 kWh d(-1) is estimated to be generated from such AD plant. Therefore, producing biomethane from SHW can significantly promote bioenergy production in the US, thus enhancing the quick reach of bioenergy development targets set at the Federal and State levels, while overcoming the technical and management barriers of using SHW.