This study reports the synthesis of a nanocomposite comprised of graphene (G) supported manganese dioxide (MnO2) incorporated into the network of polythioaniline (MnO2-G/PTA). The hybrid composite was applied as an electrode material for the development of a bioanode. The bioanode was fabricated by the electrochemical entrapment of ferritin (Frt) as mediator and glucose oxidase (GOx) enzyme in the matrix of the as-synthesized MnO2-G/ PTA deposited on glassy carbon electrode (GCE) surface. The structural features and electrochemical behaviour of the modified electrodes were investigated by Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS). The results unfolded that the hybrid electroactive support (MnO2-G/PTA) employed for the immobilization of the enzyme (GOx) established an appropriate electrical cabling between the redox enzyme (GOx) and the electrode surface with the assistance provided by the biocompatible mediator (Frt) working to enhance the electrical signals. The developed GCE/MnO2-G/PTA/Frt/GOx bioanode attained a maximum current density of 3.68 mAcm(-2) at 35 mM glucose concentration at a scan rate of 100 mVs(-1). Thus, the MnO2-G/PTA/Frt/G0x modified electrode possesses high potential and good biocompatibility for bio-electricity production from glucose. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.