Enantioseparation of 1-phenylethanol with corn germ oil, obtained by supercritical carbon dioxide, is efficiently catalysed by Novozyme 435. The reaction has high enantioselectivity (E > 1000) and the equilibrium conversion is approx. 90% at 50 degrees C both at ambient pressure and at 20 MPa. The esters and the remaining alcohol can be separated by supercritical carbon dioxide extraction and sequential pressure drop using a pressurized and an atmospheric separator. The determination of suitable pressure and temperature conditions were supported by phase equilibria measurements and distribution ratio determination.