In the present work, the development of novel CeO2-ZrO2 nanoparticle-modified sensing platforms was described to address the oxygen dependency of lactate oxidase enzyme-based electrochemical lactate biosensors. Using engineered electrode modification, it was aimed to construct enzymatic lactate sensors capable of working in O-2-depleted environments. In this regard, CeO2-ZrO2 nanoparticles were chosen as electrode modifiers due to their high oxygen release and uptake properties. The nanoparticles were prepared using a facile chemical synthesis method and characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), Raman spectroscopy, and N-2 adsorption methods. The XRD and TEM results confirmed the successful synthesis of CeO2-ZrO2 nanoparticles and a physical surface area of 76.1 m(2) g(-1) was measured by BET method. The electrochemical performance results demonstrated that CeO2-ZrO2 nanoparticle-modified biosensors showed high sensitivities regardless of the subjected environments. In contrast to conventional enzymatic lactate biosensors, novel sensor design experienced only a slight drop in the response in O-2-depleted solutions, confirming that the O-2-susceptibility of the lactate biosensors was diminished significantly. The novel biosensor design showed a high sensitivity of 138.9 +/- 1.2 mu A. mM(-1). cm(-2) with the linear range of 2.5 mu M-0.6 mM. (C) 2018 The Electrochemical Society.