Mixed matrix membranes (MMMs) with superior structural and functional properties provide an interesting approach to enhance the separation properties of polymer membranes. As a matter of fact, MMMs combine the advantages of both components; polymeric continuous phase and nanoparticle dispersed phase. Generally, the separation performance of polymeric membranes suffers from an upper-performance limit. Hence, the incorporation of nanoparticles helps to overcome such limitations. Block copolymers such as poly(ether-block-amide) (PEBA) composed of immiscible soft ether segments as well as hard amide segments have been shown as excellent materials for the synthesis of membranes. Consequently, PEBA membranes have been extensively used in scientific research and industrial processes. It is thus aimed to provide an overview of PEBA MMMs. This review is especially devoted to summarizing the effects of nanoparticle loading on PEBA performance and properties such as selectivity, permeability, thermal and mechanical properties, and others. In addition, the preparation techniques of PEBA MMMs and solvent selection are discussed. This article also discusses the many types of nanoparticles incorporated into PEBA membranes. Furthermore, the future direction in PEBA MMMs research for separation processes is briefly predicted.