A co-crosslinked poly(ether imide) (PEI) material with excellent organic solvent resistance was synthesized using mixed amines of aliphatic ethylenediamine (EDA) and aromatic p-xylylenediamine (XDA), on which the organic solvent nanofiltration (NF) membrane was prepared via the interfacial polymerization between the monomer 1,2,4,5-benzene tetracarbonyl chloride (BTC) and the amine terminals on the co-crosslinked PEI. Structural characterizations indicate that the EDA-XDA co-crosslinking is an effective pathway to modulate the species and the amount of amine terminals on the surfaces, which can in turn affect the toplayer thickness and the surface roughness of the synthesized NF membrane. The results illustrate that the NF membrane prepared on the EDA-XDA (1:1) co-crosslinked PEI (c-P/1E1X) possesses superior separation performance and stability for N,N-di-methylformamide (DMF) solution. Further, the superior solvent resistant NF membrane gly-NF-c-P/1E1X-XDA was synthesized through an additional quick patching-like IP using XDA, with the aid of ethylene glycol filling inside the intermediate pores. The membrane gly-NF-c-P/1E1X-XDA shows the DMF permeance of 6.1 L m(-2) h(-1) MPa-1 and the Rose Bengal (RB) rejection about 89%, and for a 5 g/L ceftazidime antibiotic DMF solution, the permeance is 4.6 L m(-2) h(-1) MPa-1 and the ceftazidime rejection is 81%, which is promising to be applied for the recovery of heat sensitivity ceftazidime in DMF solution.