Enhanced lipid content in Chlorella sp. FC2 IITG via high energy irradiation mutagenesis

Muthusivaramapandian Muthuraj; Baskar Selvaraj; Basavaraj Palabhanvi; Vikram Kumar; Debasish Das

Korean Journal of Chemical Engineering, Vol.36, No.1, 63-70, January, 2019

DOI10.1007/s11814-018-0180-z Export Citation

Enhanced lipid content in Chlorella sp. FC2 IITG via high energy irradiation mutagenesis

Muthusivaramapandian Muthuraj¹, Baskar Selvaraj¹, Basavaraj Palabhanvi¹, Vikram Kumar², and Debasish Das^{1, 2, †}

¹Department of Biosciences & Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India
²Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India

E-mail:

High energy ultra-violet radiation was used to induce mutations in the wild type strain Chlorella sp. FC2 IITG and the mutants obtained were screened for enhanced lipid content targeted towards biodiesel production. Screening of mutants under nitrogen starved photoautotrophic condition showed five mutant strains with higher lipid content as compared to the wild type strain. Maximum neutral lipid content of 22.26% (w/w, DCW) was found for FC2-25UV mutant strain, which was 48.4% higher than that of wild type culture. Further characterization under photoautotrophic two stage high cell density cultivation of lipid rich FC2-25UV resulted in total lipid content of 68% (w/w, DCW), which was 21.43% higher than the wild type strain with a marginal improvement of 11% in the total lipid productivity. Comparison of enzyme activity assays under nitrogen starved conditions in both the strains revealed an 18.2% and 31.25% increase in acetyl CoA carboxylase and di-acyl-glycerol transferase activity of mutant. The FAME composition analysis showed 41.5% and 24% increase in the fractions of C18:2 and C18:1 in the mutant strain when compared to the wild type strain with no significant difference in the other FAME fractions. Thus, the mutant strain could be a potential candidate for biodiesel production.

Keywords:Microalgae;Mutation;Ultraviolet Rays;High Cell Density Cultivation;Lipid Content

[References]

Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR, Biotechnol. Bioeng., 102(1), 100 (2009)
Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A, Plant J., 54, 621 (2008)
Kumar V, Muthuraj M, Palabhanvi B, Ghoshal AK, Das D, Renew. Energy, 68, 560 (2014)
Seth JR, Wangikar PP, Biotechnol. Bioeng., 112(7), 1281 (2015)
Anandarajah K, Mahendraperumal G, Sommerfeld M, Hu Q, Appl. Energy, 96, 371 (2013)
Beacham TA, Macia VM, Rooks P, White DA, Ali ST, Biotechnol. Rep., 7, 87 (2015)
Ma YB, Wang ZY, Zhu M, Yu CJ, Cao YP, Zhang DY, Zhou GK, Bioresour. Technol., 136, 360 (2013)
Bougaran G, Rouxel C, Dubois N, Kaas R, Grouas S, Lukomska E, Le Coz JR, Cadoret JP, Biotechnol. Bioeng., 109(11), 2737 (2012)
Vigeolas H, Dubya F, Kaymak E, Niessena G, Motteb P, Franck F, Remacle C, J. Biotechnol., 162, 3 (2012)
Rowlands RT, Enzyme Microb. Technol., 6, 3 (1984)
Mehtani J, Arora N, Patel A, Jain P, Pruthi PA, Poluri KM, Pruthi V, Bioresour. Technol., 242, 121 (2017)
Shaish A, Ben-Amotz A, Avron M, J. Appl. Phycol., 27, 652 (1991)
Alonso D, del Castillo CS, Grima EM, Cohen Z, J. Phycol., 32, 339 (1996)
Kao CY, Chiu SY, Huang TT, Dai L, Wang GH, Tseng CP, Chen CH, Lin CS, Biomass Bioenerg., 36, 132 (2012)
Palabhanvi B, Kumar V, Muthuraj M, Das D, Bioresour. Technol., 173, 245 (2014)
Muthuraj M, Chandra N, Palabhanvi B, Kumar V, Das D, BioEnergy Res., 8, 726 (2015)
Muthuraj M, Kumar V, Palabhanvi B, Das D, J. Ind. Microbiol. Biotechnol., 41, 499 (2014)
Singh PK, Arch. Microbiol., 103, 297 (1975)
Wybenga DR, Giorgio JD, Pileggi VJ, Clin Chem., 17, 891 (1971)
Parsons TR, Maita Y, Lalli CM, A manual of chemical and biological methods for sea water analysis, Pergamon Press Ltd., Great Britain (1984).
Tillich UM, Lehmann S, Schulze K, Duhring U, Frohme M, PLoS ONE, 7, e49467 (2012).
Zayadan BK, Purton S, Sadvakasova AK, Userbaeva AA, Bolatkhan K, Russ. J. Plant Physiol., 61, 124 (2014)
Wang JF, Li RM, Ma S, Li WJ, World J. Microbiol. Biotechnol., 25, 921 (2009)
Karemore A, Pal R, Sen R, Algal. Res., 2, 113 (2013)
Doan TTY, Obbard JP, Algal. Res., 1, 17 (2012)
Sachdeva N, Gupta RP, Mathur AS, Tuli DK, Bioresour. Technol., 221, 576 (2016)
Zhang Y, He ML, Zou SM, Fei C, Yan YQ, Zheng SY, Rajper AA, Wang CH, Bioresour. Technol., 207, 268 (2016)
Patel VK, Maji D, Pandey SS, Rout PK, Sundaram S, Kalra A, Algal. Res., 16, 36 (2016)
Muthuraj M, Palabhanvi B, Misra S, Kumar V, Sivalingavasu K, Das D, Photosynth. Res., 118, 167 (2013)
Radakovits R, Jinkerson RE, Darzins A, Posewitz MC, Eukaryot. Cell., 9, 486 (2010)
Ma X, Liu J, Liu B, Chen T, Yang B, Chen F, Algal. Res., 16, 28 (2016)
Che RQ, Huang L, Xu JW, Zhao P, Li T, Ma HX, Yu XY, Bioresour. Technol., 227, 324 (2017)
Fan J, Cui Y, Wan M, Wang W, Li Y, Biotechnol. Biofuels, 7(17), 1 (2014)
Morales-Sanchez D, Kim Y, Teng EL, Peterson L, Cerutti H, Plant J., 90, 1079 (2017)

[Referenced By]

[1] Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR, Biotechnol. Bioeng., 102(1), 100 (2009)

[2] Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A, Plant J., 54, 621 (2008)

[3] Kumar V, Muthuraj M, Palabhanvi B, Ghoshal AK, Das D, Renew. Energy, 68, 560 (2014)

[4] Seth JR, Wangikar PP, Biotechnol. Bioeng., 112(7), 1281 (2015)

[5] Anandarajah K, Mahendraperumal G, Sommerfeld M, Hu Q, Appl. Energy, 96, 371 (2013)

[6] Beacham TA, Macia VM, Rooks P, White DA, Ali ST, Biotechnol. Rep., 7, 87 (2015)

[7] Ma YB, Wang ZY, Zhu M, Yu CJ, Cao YP, Zhang DY, Zhou GK, Bioresour. Technol., 136, 360 (2013)

[8] Bougaran G, Rouxel C, Dubois N, Kaas R, Grouas S, Lukomska E, Le Coz JR, Cadoret JP, Biotechnol. Bioeng., 109(11), 2737 (2012)

[9] Vigeolas H, Dubya F, Kaymak E, Niessena G, Motteb P, Franck F, Remacle C, J. Biotechnol., 162, 3 (2012)

[10] Rowlands RT, Enzyme Microb. Technol., 6, 3 (1984)

[11] Mehtani J, Arora N, Patel A, Jain P, Pruthi PA, Poluri KM, Pruthi V, Bioresour. Technol., 242, 121 (2017)

[12] Shaish A, Ben-Amotz A, Avron M, J. Appl. Phycol., 27, 652 (1991)

[13] Alonso D, del Castillo CS, Grima EM, Cohen Z, J. Phycol., 32, 339 (1996)

[14] Kao CY, Chiu SY, Huang TT, Dai L, Wang GH, Tseng CP, Chen CH, Lin CS, Biomass Bioenerg., 36, 132 (2012)

[15] Palabhanvi B, Kumar V, Muthuraj M, Das D, Bioresour. Technol., 173, 245 (2014)

[16] Muthuraj M, Chandra N, Palabhanvi B, Kumar V, Das D, BioEnergy Res., 8, 726 (2015)

[17] Muthuraj M, Kumar V, Palabhanvi B, Das D, J. Ind. Microbiol. Biotechnol., 41, 499 (2014)

[18] Singh PK, Arch. Microbiol., 103, 297 (1975)

[19] Wybenga DR, Giorgio JD, Pileggi VJ, Clin Chem., 17, 891 (1971)

[20] Parsons TR, Maita Y, Lalli CM, A manual of chemical and biological methods for sea water analysis, Pergamon Press Ltd., Great Britain (1984).

[21] Tillich UM, Lehmann S, Schulze K, Duhring U, Frohme M, PLoS ONE, 7, e49467 (2012).

[22] Zayadan BK, Purton S, Sadvakasova AK, Userbaeva AA, Bolatkhan K, Russ. J. Plant Physiol., 61, 124 (2014)

[23] Wang JF, Li RM, Ma S, Li WJ, World J. Microbiol. Biotechnol., 25, 921 (2009)

[24] Karemore A, Pal R, Sen R, Algal. Res., 2, 113 (2013)

[25] Doan TTY, Obbard JP, Algal. Res., 1, 17 (2012)

[26] Sachdeva N, Gupta RP, Mathur AS, Tuli DK, Bioresour. Technol., 221, 576 (2016)

[27] Zhang Y, He ML, Zou SM, Fei C, Yan YQ, Zheng SY, Rajper AA, Wang CH, Bioresour. Technol., 207, 268 (2016)

[28] Patel VK, Maji D, Pandey SS, Rout PK, Sundaram S, Kalra A, Algal. Res., 16, 36 (2016)

[29] Muthuraj M, Palabhanvi B, Misra S, Kumar V, Sivalingavasu K, Das D, Photosynth. Res., 118, 167 (2013)

[30] Radakovits R, Jinkerson RE, Darzins A, Posewitz MC, Eukaryot. Cell., 9, 486 (2010)

[31] Ma X, Liu J, Liu B, Chen T, Yang B, Chen F, Algal. Res., 16, 28 (2016)

[32] Che RQ, Huang L, Xu JW, Zhao P, Li T, Ma HX, Yu XY, Bioresour. Technol., 227, 324 (2017)

[33] Fan J, Cui Y, Wan M, Wang W, Li Y, Biotechnol. Biofuels, 7(17), 1 (2014)

[34] Morales-Sanchez D, Kim Y, Teng EL, Peterson L, Cerutti H, Plant J., 90, 1079 (2017)