On-demand Microfluidic Manipulation of Thermally Stable Water-in-Perfluorocarbon Emulsions

Mun-Bae Jang; Taewoo Moon; Jae-Hoon Choi; Sung-Hee Chung; Jong-Wook Ha; Jong-Min Lim; Seung-Kon Lee; Seung-Man Yang; Kyung-Ho Youm; Kwanwoo Shin; Gi-Ra Yi

Macromolecular Research, Vol.26, No.12, 1160-1166, December, 2018

DOI10.1007/s13233-018-6144-7 Export Citation

On-demand Microfluidic Manipulation of Thermally Stable Water-in-Perfluorocarbon Emulsions

Mun-Bae Jang, Taewoo Moon¹, Jae-Hoon Choi², Sung-Hee Chung¹, Jong-Wook Ha³, Jong-Min Lim^{4, †}, Seung-Kon Lee⁵, Seung-Man Yang², Kyung-Ho Youm, Kwanwoo Shin^{1, †}, and Gi-Ra Yi^{6, †}

Department of Engineering Chemistry, Chungbuk National University, Cheongju, Chungbuk 28644, Korea
¹Department of Chemistry, Sogang University, Seoul 04107, Korea
²Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Korea
³Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Korea
⁴Department of Chemical Engineering, Soonchunhyang University, Asan, Chungnam 31538, Korea
⁵Korea Atomic Energy Research Institute (KAERI), Daejeon 34101, Korea
⁶School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi 16419, Korea

E-mail:, ,

Monodispersed thermally stable aqueous emulsions were generated in perfluorocarbon oil with penta-block copolymers of fluorocarbon and poly(ethylene oxide-b-propylene oxide-b-ethylene oxide), which are controlled in guided channels of microfluidic devices, which were essentially the protrusion on top of microfluidic channels. Aqueous emulsions could stably follow the guide channels and sequentially stored at dead ends of guide channels. Depending on the size ratio of emulsion to trapping region, one or two emulsions were stored in two-dimensional arrays on demand. By adding pneumatic valve actuators on top of guide channel, they could be stored or released on demand or as programmed. Furthermore, by encapsulating colloidal particles and Hela cells in the thermally stable water-in-perfluorocarbon emulsions, we demonstrated that particle-laden and cellladen emulsions could be manipulated in controlled and programmed manner.

Keywords:microfluidic chip;emulsions;pneumatic valves;on-demand manipulation;hela cells

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[2] Stone HA, Stroock AD, Ajdari A, Ann. Rev. Fluid Mech., 36, 381 (2004)

[3] Squires TM, Quake SR, Rev. Mod. Phys., 77, 977 (2005)

[4] Brouzes E, Medkova M, Savenelli N, Marran D, Twardowski M, Hutchison JB, Rothberg JM, Link DR, Perrimon N, Samuels ML, Proc. Natl. Acad. Sci, USA, 106, 14195 (2009)

[5] Miller OJ, Harrak AE, Mangeat T, Baret JC, Frenz L, Debs BE, Mayot E, Samuels ML, Rooney EK, Dieu P, Galvan M, Link DR, Griffiths AD, Proc. Natl. Acad. Sci, USA, 109, 378 (2011)

[6] Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD, Nat. Methods, 3, 545 (2006)

[7] Zhong Q, Bhattacharya S, Kotsopoulos S, Olson J, Taly V, Griffiths AD, Link DR, Larson JW, Lab Chip, 11, 2167 (2011)

[8] Boybay MS, Jiao A, Glawdel T, Ren CL, Lab Chip, 13, 3840 (2013)

[9] Leung K, Zahn H, leaver T, Konwar KM, Hanson NW, Page AP, Lo CC, Chain PS, Hallam SJ, Hansen CL, Proc. Natl. Acad. Sci, USA, 109, 7665 (2012)

[10] Shim JU, Cristobal G, Link DR, Thorsen T, Jia YW, Piattelli K, Fraden S, J. Am. Chem. Soc., 129(28), 8825 (2007)

[11] Boukellal H, Selimovic S, Jia Y, Cristobal G, Fr S, Lab Chip, 9, 331 (2009)

[12] Fradet E, McDougall C, Abbyad P, Dangla R, McGloin D, Baroud CN, Lab Chip, 11, 4228 (2011)

[13] Abbyad P, Dangla R, Alexandrou A, Baroud CN, Lab. Chip, 11, 813 (2011)

[14] Sochol RD, Li S, Lee LP, Lin L, Lab Chip, 12, 4168 (2012)

[15] Urbanski JP, Thies W, Rhodes C, Amarasinghe S, Thorsen T, Lab Chip, 6, 96 (2006)

[16] Choi JH, Lee SK, Lim JM, Yang SM, Yi GR, Lab Chip, 10, 456 (2010)

[17] Holtze C, Rowat AC, Agresti JJ, Hutchison JB, Angile FE, Schmitz CHJ, et al., Lab Chip, 8, 1632 (2008)

[18] Tewhey R, Warner JB, Nakano M, Libby B, Medkova M, Da PH, Nat. Biotechnol., 27, 1025 (2009)

[19] Zeng Y, Novak R, Shuga J, Smith MT, Mathies RA, Anal. Chem., 82, 3183 (2010)

[20] Zhu Z, Jenkins G, Zhang W, Zhang M, Guan Z, Yang CJ, Anal. Bioanal. Chem., 403, 2127 (2012)

[21] Zhang H, Jenkins G, Zou Y, Zhu Z, Yang CJ, Anal. Chem., 84, 3599 (2012)

[22] Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, et al., Anal. Chem., 83, 8604 (2011)

[23] Pinheiro LB, Coleman VA, Hindson CM, Herrmann J, Hindson BJ, Bhat S, Emslie KR, Anal. Chem., 84, 1003 (2012)