Supercritical CO2 was used to fractionate a mixture of monoacylglycerols (MAGs), diacylgylcerols (DAGs) and triacylglycerols (TAGs) using an eight-foot packed column which was kept under thermal gradient conditions and operated semi-continuously. Fractionation of the glyceride mixtures was affected by both their solubility in CO2 and respective vapor pressure enhancement. The feed material used, approximating a feed stream used in the industrial enrichment of MAGs, was composed of 48.7 wt.% MAG, 46.3 wt.% DAG and 5 wt.% TAG, having main acyl chain compositions of 6 wt.% C16:0, 23 wt.% C18:0 and 59 wt.% C18:1. The resultant top product from the fractionation column had MAG concentrations as high as 90 wt.% and no TAG, which is comparable to that produced by molecular distillation. The effects of pressure, temperature gradient, CO2 flow rate and feed composition on the separation efficiency and yields were investigated. Increasing the column pressure from 172 to 344 bar or the density of CO2 from 420 to 720 kg m(-3) increased the yield of the top product. However, with those increases, the supercritical fluid became less selective, with the MAG concentration decreasing from 84 to 56 wt.%. At 207 bar, a linear temperature gradient varying from 65 to 95 degrees C gave the best selectivity when compared to other temperature gradient schemes. Increasing the CO2 flux from 1.8 to 10.3 g min(-1) cm(-2) resulted in little change in the MAG concentration, but the top-product yield decreased from 14.4 to 2.3 g kg(-1) CO2. As the MAG concentration in the feed mixture increased from 48.7 to 63 wt.%, the MAG concentration in the top product (at 207 bar and 65-95 degrees C) increased from 79.4 to 86 wt.%.