In this study, a large-scale plug flow reactor has been operated at a hydraulic retention time of 25 d with substrate concentration of total solids (7-10%) under a temperature of 37-40 degrees C with a working volume of 3.85x10(4) m(3) and dispose 1504m(3)/d of waste for three years. The average monthly biogas production was 7.45x10(4) m(3). At stable stage, the volume biogas, methane production rate and methane content reached 1.07m(3)/m(3.)d, 0.58m(3)/m(3).d and 56.4%, respectively. Moreover, the specific biogas and methane yields of substrate removal efficiency were up to 0.39m(3)/kg VS, 0.22m(3)/kg VS and 59%, respectively. Among the bacteria, the genera Draconibacteriaceae, Ruminofilibacter, Cloacimonetes and vadinBC27, Ruminiclostridium and Treponema_2 dominated in digestate. The achaeal was dominated by methanogens genera Methanosarcina and Bathyarchaeota. Long-term operation in large scale demonstrated technological potential and industrial application of cattle manure treatment for biogas production.