In this study, an attempt was made to enhance the biomethanation potential of seagrass (Syringodium isoetifolium) by the aid of disperser-tenside (polysorbate 80) disintegration for the first time in literature. A disperser rpm of 10,000 for 20 min and PS 80 dose of 0.000864 g/g TS were selected as ideal parameters for effectual seagrass biomass disintegration. Dispersion aided tenside disintegration (DTD) with a disperser energy consumption of 349 kJ/kg TS, was observed to be efficacious with a biomass lysis rate of 25.6%. The impact of DTD on bioacidification and biomethanation assay with respect to volatile fatty acids concentration (1100 mg/L) and methane generation (0.256 g/g COD), was greater than dispersion disintegration (DD) (800 mg/L; 0.198 g/g COD). Thus, S. isoetifolium is considered as a promising substrate to attain the third generation biofuel goals in the near future.