This study investigated the feasibility of dry anaerobic digestion using beer lees as substrate and the effect of cow manure-derived biochar addition on dry anaerobic digestion performance at mesophilic and thermophilic temperature, respectively. With TS content of 25%, maximum cumulative methane production and yield were achieved to be 5230 +/- 91 mL d(-1) and 220.1 +/- 7.7 mL g(-1) VS at mesophilic condition and 7386 +/- 134 mL d(-1) and 310.4 +/- 9.2 mL g(-1) VS at thermophilic condition in the control cultures. The biochar addition has a positive effect in improving dry anaerobic digestion performance. The maximum cumulative methane production and yield in the cultures with 10 g L-1 biochar were substantially improved by 82.9% and 82.6% at mesophilic condition and 47.2% and 46.8% at mesophilic condition when compared to the control.