Utilization of sustainable energy is limited by energy requirement for the manufacturing of renewable fuels. Moso bamboo was pretreated with industrially derived crude glycerol obtained from different sources at 150/160 degrees C for 3 h. This bamboo, pretreated with base biodiesel glycerol with pressure filtration removal method, showed a high glucose yield of 94.95% and an ethanol yield of 73.10% of the theoretical. Major glycerol content was removed by pressure filtration, leaving a small amount of fatty acid soap in the pretreated sample, which formed an emulsion that reduced lignin redisposition onto the biomass surface and effectively blocked lignin absorption of cellulase, allowing greater enzymatic hydrolysis and fermentation system function. The surface was more hydrophilic and a higher lignin removal was achieved: 39.24% with base biodiesel glycerol pretreatment compared to 26.08% with sodium hydroxide glycerol pretreatment. This study provides a useful and cost-effective process, BBGP, for high-yield ethanol production.