This work investigated the thermophilic (55 degrees C)Co-digestion performance both in batch andContinuous mode operation. The biochemical methane potentials of L. digitata andCattle manure were 308 +/- 24 and 203 +/- 33 mLCH(4)/g VS, respectively. The optimumCo-digestion feedstock ratio was found to be 80% macroalgae: 20% manure on a volatile solids basis, which produced 290 +/- 19 mLCH(4)/g VS under long-term and stableContinuous operation at an organic loading rate of 2 g VS/L/d and hydraulic retention time of 15 days. Simulations of the batch andContinuous experiments were, for the first time,Carried out using an integrated anaerobic bioconversion model without structural modifications.Close fits between measured and simulated data provided mutualConfirmation of experimental reliability and model robustness, and provided new perspectives for the use of the software tool.