An integrated production of methyl ester and.-polylysine from Chlamydomonas sp. was studied using biorefinery approach. The harvesting efficiency of Chlamydomonas sp. was increased up to 92% by treatment with a flocculant FeCl3 at 100 mg/L for 30 min. The DMC (dimethyl carbonate) mediated enzyme catalyzed in-situ transesterification of Chlamydomonas sp. yielded the maximum methyl ester of 92% under optimized conditions. The valued-added product.-polylysine was produced from hydrolysate obtained from the spent biomass of Chlamydomonas sp. using Streptomyces sp. The key components of sugar and MgSO4 used for epsilon-polysine production were optimized whereby the maximum epsilon-polylysine production was achieved at 50 g/L sugar and 0.3 g/L MgSO4. The.-polylysine production was further enhanced by supplementation of important amino acids (lysine and aspartate) and TCA cycle intermediates (citric acid and a-ketoglutaric acid). The maximum epsilon-polylysine production of 2.24 g/L was found with 4mM citric acid supplementation after 110 h.