Nanotechnology has recently attracted growing interest in the encapsulation of botanical insecticides for sustainable pestControl. Biocompatible, biodegradable, and stimuli-responsive devices, however, were scarcely reported in the field of intelligent formulations of botanical insecticides. In the present study, a temperature-responsive mixed micelle (MMs-Pys-7) was developed for Controlled release of pyrethrins by Cooperative assembly of poly[2-(2-Methoxyethoxy) ethyl methacrylate-co-Octadecyl methacrylate] (P(MEO(2)MA(94)-co-ODA(6))), monomethoxy (polyethylene glycol) 13-poly(D, L-Lactide-co-glycolide) (mPEG(13)-b-PLGA(5-3)) and monomethoxy (polyethylene glycol) 45-poly(D, L-Lactide) (mPEG(45)-b-PLA(28)) in water. This nanodeviceCould protect the encapsulated pyrethrins from ultraviolet degradation at 26 degrees C effectively. It also underwent a phase transition process in water from solution state to turbid state with temperature rising from 13 to 26 degrees C. By inducing this structural Change, MMs-Pys-7 Could regulate the pyrethrin release amounts to adapt to the larval mosquito population variations along with the ambient temperature Changes. Therefore, MMs-Pys-7 exhibited a higher larvicidal activity against Culex pipiens pallens at 26 degrees C than that at 14 degrees C or 18 degrees C. Compared with a Commercial pyrethrin formulation (EW-Pys), MMs-Pys-7 was more toxic to C. p. pallens larvae at 26 degrees C after 24-h exposure but less toxic at 14 degrees C. Moreover, MMs-Pys-7 also exhibited a longer-lasting larvicidal activity under natural conditions as Compared to EW-Pys. These results suggest that MMs-Pys-7 has great potential to be developed as an intelligent nano-insecticide for mosquito control and that the controlled release mode established in this study can be applied to develop more smart formulations of botanical insecticides of application potential.