It was well-known that Morchella esculenta has a life cycle including vegetative hyphae, sclerotia, primordia, and fruiting bodies, but there is no report yet about the influence of mycelial mass on fruiting process. Since 2014, we have developed an ELISA method to detect the content of Morchella esculenta. In this study, we utilized this method to measure the mycelia content, and find the correlation between mycelial content and fruiting in the wild. The study demonstrated the changes of mycelial concentration at different location around fruiting spot.