In the present work, the oxidation stabilities of oil biodiesel fuel from Jatropha curcas L were investigated by using both Rancimat and PetroOXY test method. Three types of conventional antioxidants, i.e. butylated hydroxytoluene, 6,6-di-tert-butyl-2,2-methylenedi-p-cresol, and commercialized amine, were employed and the oxidation stability effects of those additives were examined. Oxidation stability was influenced differently depending on the type of antioxidants and their concentrations. The obtained experimental data from Rancimat and PetroOXY test methods resulting in a linear correlation at various mixtures shows the potential use of PetroOXY test method for future analytical oxidation stability test. In this research, the changes in the chemical properties of fuel sample, such as: peroxide value, acid value, composition, and kinematic viscosity, obtained from the experimental work were thoroughly discussed.