The current study investigates the combustion performance of straight algae oil (AO) in a 7-kW lab-scale gas turbine burner enabled by a novel twin-fluid injector, named Swirl-burst (SB) injector. The chemical structure (fatty acid profile), the physical, and chemical properties of AO are acquired to understand the combustibility of the oil as a potential biofuel. Effects of equivalence ratio (ER) and atomizing air to liquid mass ratio (ALR) across the injector on the global combustion characteristics are investigated at a constant heat release rate for the oil. The features of interest include visual flame images, product gas temperature, emissions of carbon monoxide (CO), and nitrogen oxides (NOx) at the combustor exit. Results show that mono-unsaturated fatty acid is predominant in the composition of the oil, suggesting possibly short ignition delay. AO has a heating value comparable to that of diesel but with a high kinematic viscosity (approximately 16 times more viscous than diesel). Clean combustion highly depends on fine sprays that lead to fast fuel pre-vaporization, thorough fuel-air mixing, and thus clean premixed combustion. Conventional injectors such as air blast (AB) atomizers cannot finely atomize viscous oils for clean combustion due to the low viscosity tolerance. Fortunately, with the SB injection, clean, complete, and lean-premixed combustion of straight AO has been successfully achieved without fuel preheating at most of the investigated cases, reasonably reflecting the fine atomization capability of the SB injector even for the viscous oil. The blue flames, overlapping temperature profiles, and low emissions of CO and NOx consistently show the clean lean-premixed AO flames. Stable and clean flames are obtained at ERs of 0.60-0.75 (with the optimum ER identified at 0.65, in terms of flame stability and low emissions), and a blowout limit at the ER of about 0.55. At the ER of 0.65, clean and lean-premixed flames are also acquired for all the tested ALRs (2.0-5.0). Increase in ALR varies the SAA and spray behavior of the SB injection as well as the aerodynamic interaction between fuel and oxidizer. The increasing ALR results in dominantly blue flames and decrease in CO and NOx concentrations, less than 10 ppm at ALRs > 2.5, due to finer atomization and better fuel-air mixing at the higher ALRs, and thus enhanced premixed combustion. Overall, clean and stable leanpremixed combustion of straight AO is achieved without fuel preheating using the novel SB injector despite the high fuel viscosity. The SB injection potentially enables AO itself as a cost-effective and near-zero-emission biofuel.