The technical feasibility of commercial gas production from microbially enhanced coalbed methane (MECBM) reservoirs can be divided into two broad categories: microbial treatment procedures and reservoir suitability. At this time, studies investigating the changes in reservoir-scale properties of coal as a result of bioconversion are lacking. This paper, first of a two-part series, presents the experimental work aimed at investigating the changes in sorption-induced strain response of coal to helium and methane flooding as a result of bioconversion. The strain and its variation, critical to producing methane after bioconversion, were established for coal treated for three periods, 15, 30 and 60 days. The results indicated a softer coal post-bioconversion which, in turn, resulted in inelastic strain with initial helium injection. However, past this initial compression, the bioconverted coal behavior was similar to untreated coal during continued helium injection/depletion. Methane flooding resulted in decreased sorption-induced shrinkage post-treatment, the decrease in Langmuir pressure and volume constants becoming larger with continued treatment. The observed results were used to understand the changes in coal structure for production of biogenic coalbed methane (BCBM) from depleted CBM reservoirs, recharged with methane using the bioconversion technology. The second part presents a detailed analysis of the experimental results to evaluate the behavior and performance of BCBM reservoirs.